Isolation of chlamydiae in untreated and Cytochalasin B treated McCoy cells.
نویسندگان
چکیده
منابع مشابه
Growth of Chlamydia trachomatis in McCoy cells treated with cytochalasin B.
When the fungal metabolite cytochalasin B was added to McCoy cells, multinucleated giant cells developed. Monolayers of these cells proved as efficient as irradiated cells for the growth of three different serotypes of Chlamydia trachomatis and for the primary isolation of chlamydiae from clinical specimens obtained from patients attending a venereal disease clinic. Cytochalasin treatment of Mc...
متن کاملIsolation of chlamydia in irradiated and non-irradiated McCoy cells.
Specimens from eye and genital tract were cultured in parallel in irradiated and non-irradiated McCoy cells and the frequency of isolation of chlamydia using these culture methods was compared. There was a significant difference between the frequencies of isolation; irradiated McCoy cells produced a greater number of positive results.
متن کاملCultivation of Chlamydia trachomatis in cycloheximide-treated mccoy cells.
An isolation technique for Chlamydia trachomatis using McCoy cells is described. In contrast to earlier techniques employing such cells, no pretreatment of the cells was used. The glutarimide antibiotic cycloheximide was added to the culture medium used for incubating the cells after infection. Cycloheximide was used at concentrations that depressed, but did not completely inhibit, the metaboli...
متن کاملCytochalasin B and the Adenosine Triphosphate Content of Treated Fibroblasts
INTRODUCTION the addition of the drug . Concentrations of about 3 μg/ml were required to observe altered cell morphology. The possibility was considered that depolymerization of microfilaments-with a resulting change in cell shape and function--resulted from a rapid depletion of intracellular energy levels brought about by the inhibition of sugar transport . The effects of cytochalasin B and io...
متن کاملSite-specific Treated with Maturation of Enveloped Cytochalasin B Viruses in L Cells
Treatment of infected L cells with 10 /~g/ml cytochalasin B (CB) was found to promote a rapid relocalization of viral glycoproteins on the cell surface. Whereas the vesicular stomatitis virus G protein and the influenza virus hemagglutinin were uniformly distributed on the surface of untreated cells, in CB-treated cells, they were strikingly concentrated at cell extremities in the regions of cl...
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ژورنال
عنوان ژورنال: Journal of Clinical Pathology
سال: 1978
ISSN: 0021-9746
DOI: 10.1136/jcp.31.2.183